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Up coming, we addressed cells with diverse concentrations of NAD+. When included for 24 h at 80 µM in HeLa and four hundred µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Extended Details Fig. 1k,l). Also, sixteen µM of NAD+ was ample to cut back fork pace by ~forty% in HeLa cells without the need of affecting the